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Rat Pulmonary Artery Smooth Muscle Cells
Cat.No.: CSC-C4138X
Species: Rat
Source: Lung; Artery
Cell Type: Smooth Muscle
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Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin.
Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray.
Repeated freezing and thawing of cells is not recommended.
Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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Description: Bioarray offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request.
Description: Characterization: 1. Cell Activity;
- Recovery Viability ≥ 80%.
2. Cell Purity;
- GFAP-positive cells ≥ 70%.
- β-tubulin III- or doublecortin- (Dcx-) positive cells ≤ 10%.
- GalC-positive cells ≤ 10%.
Description: Characterization: 1. Cell Activity;
- Recovery Viability ≥ 50%.
- After approximately 2 days in culture, the cells will form clear dendrites and axons.
- Cells can survive in culture for as long as 13-16 days.
2. Cell Purity;
- MAP2-positive cells ≥ 70%.
- β-tubulin III-positive cells ≥ 70%.
Description: Characterization: 1. Cell Activity;
- Recovery Viability ≥ 50%
- After approximately 2 days in culture, the cells will form clear dendrites and axons.
- Cells can survive in culture for as long as13-16 days.
2. Cell Purity;
- MAP2-positive cell percentage ≥ 70%.
- β-tubulin III-positive cell percentage ≥ 70%.
Description: Characterization: 1. Cell Activity;
Recovery Viability ≥ 80%.
2. Cell Purity;
- GFAP-positive cells ≥ 70%.
- β-tubulin III- or doublecortin- (Dcx-) positive cells ≤ 10%.
- GalC-positive cells ≤ 10%.
Description: Characterization: 1. Cell Activity;
- Recovery Viability ≥ 50%.
- After approximately 2 days in culture, the cells will form clear dendrites and axons.
- Cells can survive in culture for as long as 13-16 days.
2. Cell Purity;
- MAP2-positive cells ≥ 70%.
- β-tubulin III-positive cells ≥ 70%.
