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Rat Primary Spleen Epithelial Cells
Cat.No.: CSC-C4148X
Species: Rat
Source: Spleen
Cell Type: Epithelial
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Rat Spleen Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Spleen Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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Description: Bioarray offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request.
Description: Characterization: 1. Cell Activity;
- Recovery Viability ≥ 80%.
2. Cell Purity;
- GFAP-positive cells ≥ 70%.
- β-tubulin III- or doublecortin- (Dcx-) positive cells ≤ 10%.
- GalC-positive cells ≤ 10%.
Description: Characterization: 1. Cell Activity;
- Recovery Viability ≥ 50%.
- After approximately 2 days in culture, the cells will form clear dendrites and axons.
- Cells can survive in culture for as long as 13-16 days.
2. Cell Purity;
- MAP2-positive cells ≥ 70%.
- β-tubulin III-positive cells ≥ 70%.
Description: Characterization: 1. Cell Activity;
- Recovery Viability ≥ 50%
- After approximately 2 days in culture, the cells will form clear dendrites and axons.
- Cells can survive in culture for as long as13-16 days.
2. Cell Purity;
- MAP2-positive cell percentage ≥ 70%.
- β-tubulin III-positive cell percentage ≥ 70%.
Description: Characterization: 1. Cell Activity;
Recovery Viability ≥ 80%.
2. Cell Purity;
- GFAP-positive cells ≥ 70%.
- β-tubulin III- or doublecortin- (Dcx-) positive cells ≤ 10%.
- GalC-positive cells ≤ 10%.
Description: Characterization: 1. Cell Activity;
- Recovery Viability ≥ 50%.
- After approximately 2 days in culture, the cells will form clear dendrites and axons.
- Cells can survive in culture for as long as 13-16 days.
2. Cell Purity;
- MAP2-positive cells ≥ 70%.
- β-tubulin III-positive cells ≥ 70%.
