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Primary Human Fetal Lung Fibroblast Cells
Cat.No.: CSC-C4382X
Species: Human
Source: Lung
Cell Type: Fibroblast
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These cells were originated using Complete Serum-Free Medium Kit With SuperFuel™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5 X 10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.
These cells are available in both cryopreserved vials as well as in 25cm2 and 75cm2 proliferating culture flasks.
Each vial or flask of cells is shipped to Customer with Bac-O (antibiotic) and SuperEnergy™ (animal derived growth factors) or SuperEnergy-R™ (human recombinant growth factors) at no additional cost.
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Description: HTSMC from Creative Bioarray Research are isolated from human trachea. HTSMC are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HTSMC are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin and desmin. HTSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTSMC are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Description: HPAAF from Creative Bioarray are isolated from human pulmonary artery. HPAAF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPAAF are characterized by spindle morphology and by immunofluorescent method with antibody to fibronectin. HPAAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAAF are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Description: HBSMC from Creative Bioarray Research Laboratories are isolated from human bronchi and bronchioles. HBSMC are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBSMC are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin and desmin. HBSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBSMC are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Description: Human Pulmonary Artery Smooth Muscle Cells (HPASMCs) provided by Creative Bioarray are isolated from the normal human pulmonary artery tissue. The cells are cryopreserved at passage 2 and delivered frozen. Each vial contains more than 0.5*10^6 viable cells after thawing. The cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPASMCs are guaranteed for at least 12 population doublings under the conditions provided by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Description: Creative Bioarray's normal Human Lung Smooth Muscle Cells, when grown in SMC Medium, provide an ideal culture model for the study of angiogenesis, atherosclerosis, diabetes or vascular/pulmonary biology.
Cell Features:
HSMC are cryopreserved as secondary cells, e.g. cells are isolated from the stated tissue and expanded in culture vessels twice before cryopreservation.
HAoSMC are isolated from human aorta (ascending and/or descending).
HPASMC are isolated from human pulmonary artery.
HCASMC are isolated from human coronary arteries.
HLMSC are isolated from the lobes of the lungs.
HBTSMC are isolated from the trachea and primary bronchi.
HSMC can be grown without phenol red or antimicrobials when cultured in VascuLife SMC Medium.
HSMC are extensively tested to meet quality standards and exhibit optimal performance.
Creative Bioarray guarantees performance and quality.
Description: Creative Bioarray's normal Human Lung Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.
Cell Features:
HDFn are cryopreserved as primary cells. Cells are isolated from neonatal human foreskin and expanded in culture vessels once before cryopreservation.
HDFa are cryopreserved as primary cells. Cells are isolated from adult human skin and expanded in culture vessels once before cryopreservation.
Human Lung Fibroblasts are cryopreserved as secondary cells cells are isolated from human lungs and expanded in culture vessels twice before cryopreservation.
Fibroblasts can be grown in serum-free, animal protein-free, phenol red-free, and antimicrobial free conditions when cultured in FibroLife Serum-Free Medium.
Fibroblasts are extensively tested to meet quality standards and exhibit optimal performance.
Creative Bioarray's Fibroblasts are suitable for use as feeder layers as xeno-free or non-xeno free cultures for human embryonic stem cells and other cell culture applications requiring feeder layers.
