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Human Pancreatic Stellate Cells (HPaSteC)
Cat.No.: CSC-7740W
Species: Human
Source: Pancreas
Cell Type: Stellate
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Description: Primary Human Pancreatic Stromal Cells were initiated by elutriation from normal human pancreatic tissue.
These cells were originated using Complete Serum-Free Medium Kit With SuperFuel™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5 X 10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.
Description: Human Pancreatic Microvascular Endothelial Cells from Creative Bioarray are isolated from human pancreatic tissue. Human Pancreatic Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
Description: Human Primary Pancreatic Fibroblasts are isolated from normal human pancreatic tissue. Human Primary Pancreatic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml.
Description: Human Pancreatic Epithelial Cells from Creative Bioarray are isolated from normal human pancreatic tissue. Human Pancreatic Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Description: Human Pancreatic Islets are isolated from the pancreas of human using Collagenase P and purified using Ficoll density gradient.
Description: GFP Expressing Human Pancreatic Microvascular Endothelial Cells (GFP-HPaMVECs) provided by Creative Bioarray are puromycin-selected from Human Pancreatic Microvascular Endothelial Cells infected with lentivirus expressing GFP. GFP-HPaMVECs are cryopreserved in passage 4 and guaranteed for at least 18 population doublings under the cell culture conditions specified by Creative Bioarray. These cells are characterized by immunofluorescence staining with antibody of VWF/Factor VIII and PECAM1. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C.
