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Human Mammary Fibroblasts
Cat.No.: CSC-C1500
Species: Human
Source: Breast
Morphology: Fibroblasts
Cell Type: Fibroblast
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HMF are isolated from human mammary tissue. HMF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HMF are characterized by immunofluorescent method with antibodies to fibronectin, CD90 and vimentin. HMF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMF are guaranteed to further expand for 15 population doublings at the conditions provided by Creative Bioarray.
Never can primary cells be kept at -20 °C.
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Description: The majority of breast cancers originate in the lobular or ductal cells of the milk-producing glands. In these structures, there are two main cell types: the inner luminal cells surrounded by basal myoepithelium. These cells types are distinct and are the precursors to various forms of breast cancer. As such, it is important to study these cell populations independently as well as in co-culture models systems. Cells have tested negative for HIV-1, HIV-2, HTLV-1, HTLV-2, HbsAG, HbcAB, HCV, and RPR.
Luminal cells line the apical surface of the normal breast duct and have secretory properties. Most breast cancers arise from luminal cells and are heterogeneous in their expression of hormone receptors and ERBB2 expression.
Description: The majority of breast cancers originate in the lobular or ductal cells of the milk-producing glands. In these structures, there are two main cell types: the inner luminal cells surrounded by basal myoepithelium. These cells types are distinct and are the precursors to various forms of breast cancer. As such, it is important to study these cell populations independently as well as in co-culture models systems. Cells have tested negative for HIV-1, HIV-2, HTLV-1, HTLV-2, HbsAG, HbcAB, HCV, and RPR.
Basal myoepithelial cell surround the luminal cells and have both contractile muscle and epithelial properties. Basal myoepithelial cells tend to dominate normal primary cultures. Additionally, basal-type breast cancers are typically estrogen and progesterone receptor negative.
Description: Human Mammary Epithelial Cells (HMECs) produced at Creative Bioarray are isolated from human mammary tissue. The cells are cryopreserved at passage 2 (P2) and delivered frozen. Each vial contains more than 0.5*10^6 viable cells after thawing. The cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMECs are guaranteed for at least 15 population doublings under the conditions provided by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Description: Creative Bioarray's normal Human Mammary Epithelial Cells, when grown in Creative Bioarray's LIMam Medium, provide an ideal serum-free culture model for many areas of research. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.
Cell Features:
HMEC are cryopreserved as tertiary cells, e.g. cells are isolated from human mammary tissue and expanded three times in culture vessels before being harvested for cryopreservation.
HMEC are extensively tested for quality and optimal performance.
Creative Bioarray guarantees performance and quality.
Description: Creative Bioarray's normal Human Mammary Epithelial Cells, when grown in Creative Bioarray's LIMam Medium, provide an ideal serum-free culture model for many areas of research. Common uses of HMEC include the stages of breast cancer development, three dimensional culture and carcinogen screening.
Cell Features:
HMEC are cryopreserved as tertiary cells, e.g. cells are isolated from human mammary tissue and expanded three times in culture vessels before being harvested for cryopreservation.
HMEC are extensively tested for quality and optimal performance.
Creative Bioarray guarantees performance and quality.
Description: Human Thymus Fibroblasts are isolated from normal human thymus tissue.
