Human Intestinal Fibroblasts (HIF)

Description: Smooth muscle contraction is the fundamental event in gastrointestinal motion. Inflammation of the human intestine causes thickening of the smooth muscle layers which results from the increases in the smooth muscle-specific actins. The increased smooth muscle actins may affect force production and further demonstrate the plasticity of smooth muscle in the inflamed intestine. Human intestinal smooth muscle cells respond to IL-1-beta and TNF-alpha stimulation by releasing IL-6, which might significantly contribute to the overall systemic inflammatory response. Knowledge of molecular mechanism that underlies the control of colorectal smooth muscle tone is essential to advance of understanding of pathophysiology of the abnormality. The availability of human rectal smooth muscle cells in culture will considerably enhance our ability to study the contractile, proliferative and connective tissue responses of the smooth muscle of the human colorectal disorders.HRSMC from Bioarray Research Laboratories are isolated from the human rectum. HRSMC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRSMC are characterized by immunofluorescent method with antibodies to Α-smooth muscle actin and desmin. HRSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRSMC are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray Research Laboratories.

Description: Smooth muscle contraction is the fundamental event in gastrointestinal motion. Although many of the biochemical mechanisms underlying the excitation-contraction coupling are not yet defined, it is known that cytosolic Ca2+ is the essential component in the coupling phenomenon. Inflammation of the human intestine causes thickening of the smooth muscle layers which results from the increases in the smooth muscle-specific actins. The increased smooth muscle actins may affect force production and further demonstrate the plasticity of smooth muscle in the inflamed intestine. Studies also show that human intestinal smooth muscle cells respond to IL-1beta and TNF-alpha stimulation by releasing IL-6, which might significantly contribute to the overall systemic inflammatory response. The availability of human intestinal smooth muscle cells in culture will considerably enhance our ability to study the contractile, proliferative and connective tissue responses of the smooth muscle of the human gastrointestinal tract.HISMC from Bioarray Research Laboratories are isolated from human intestine. HISMC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HISMC are characterized by immunofluorescent method with antibodies to Α-smooth muscle actin and desmin. HISMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HISMC are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray Research Laboratories.

Description: Endothelial cells lining the microvasculature are known to play a critical "gatekeeper" role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that intestinal microvascular endothelial cells (IMEC) exhibit a strong immune response to LPS challenge and play a critical regulatory role in gut inflammation. Pharmacological inhibition of NOS in activated HIMEC resulted in a significant increase in leukocytes binding. Gene expression profile study reveals that intestinal endothelial cells express biotinidase, which is involved in biotin recycling. The in vitro culture of HIMEC enabled scientists to perform systematic analyses of the cytokine profiles with regard to mRNA expression and protein secretion, and to compare such data with cytokine profiles concomitantly displayed by other endothelial cells.HIMEC from Bioarray Research Laboratories are isolated from human intestinal tissue. HIMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HIMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HIMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIMEC are guaranteed to further culture in the conditions provided by Bioarray Research Laboratories.

Description: Smooth muscle is responsible for the contractility of hollow organs, such as blood vessels, the gastrointestinal tract, the bladder, and the uterus. Its structure differs greatly from that of skeletal muscle. The human stomach contains three layers of muscle in its walls, the outer longitudinal, the middle circular and the inner oblique and visceral smooth muscle cells makes up all three layers along the entire organ. Smooth muscle contraction is critical to peristalsis in the human stomach and the contraction may be mediated by activation of phospholipase through two distinct mechanisms (increased intracellular Ca2+ and G protein activation) and activating PKCepsilon-dependent mechanisms. In vitro study also shows that gastric smooth muscle cells express ET and eNOS and both calcium and sodium may be involved as current carriers in the generation of the plateau potential.HGSMC from Bioarray Research Laboratories are isolated from the human stomach. HGSMC are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HGSMC are characterized by immunofluorescent method with antibodies to Α-smooth muscle actin and desmin. HGSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGSMC are guaranteed to further expand for 15 population doublings at the condition provided by Bioarray Research Laboratories.

Description: Primary Human Intestinal Microvascular Endothelial Cells were initiated by elutriation from dissociated normal human small intestine tissue.
These cells were originated using Complete Serum-Free Medium Kit With SuperFuel™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5 X 10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

Description: Primary Human Large Intestine Microvascular Endothelial Cells were initiated from normal human large intestine (colon) tissue.
These cells were originated using Complete Serum-Free Medium Kit With SuperFuel™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5 X 10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.