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- Human Retinal Pigment Epithelial Cells
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- Cynomolgus Monkey Lung Microvascular Endothelial Cells
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C57BL/6 Mouse Primary Spleen Fibroblasts
Cat.No.: CSC-C4253X
Species: Mouse
Source: Spleen
Cell Type: Fibroblast
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Mouse Primary Spleen Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
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Description: Characterization: - γ-ray irradiated.
- Can be directly used as feeder cells to maintain mouse or human ESCs in the undifferentiated state.
Description: Characterization: - γ-ray irradiated.
- Can be directly used as feeder cells to maintain mouse or human ESCs in the undifferentiated state.
Description: Mouse Neural Stem Cells (Mouse NSCs) are isolated from fetal or adult mouse brains and are maintained in an undifferentiated, proliferative state by culturing them as free-floating neurospheres in a serum-free medium optimized with growth factors. Mouse NSCs are cryopreserved at 1st passage and can be cultured and propagated for 1-2 passages prior to induction of phenotypic differentiation.
Description: Characterization: - Strong capacity to expand.
- Capable of differentiating into multiple cell types such as neurons, astrocytes, and oligodendrocytes.
Description: C57BL/6 Mouse Aortic Endothelial Cells from Creative Bioarray are isolated from aorta of pathogen-free laboratory mice. C57BL/6 Mouse Aortic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Description: C57BL/6 Mouse Brain Microvascular Endothelial Cells from Creative Bioarray are isolated from brain tissue of pathogen-free laboratory mice. C57BL/6 Mouse Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
