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C57BL/6 Mouse Primary Lung Fibroblasts
Cat.No.: CSC-C1880
Species: Mouse
Source: Lung
Morphology: Multipolar
Cell Type: Fibroblast
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Cat.No.
CSC-C1880
Description
C57BL/6 Mouse Primary Lung Fibroblasts from Creative Bioarray are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Lung Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 5x10^5 cells per ml and is delivered frozen.
Species
Mouse
Source
Lung
Recommended Medium
SuperCult® Murine Fibroblast Medium
Morphology
Multipolar
Applications
Pathogenesis of pulmonary fibrosis;
Cell Type
Fibroblast
Disease
Normal
Quality Control
Mouse Primary Lung Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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