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B129 Mouse Small Intestinal Microvascular Endothelial Cells

Cat.No.: CSC-C8273W

Species: Mouse

Source: Small Intestine; Intestine

Cell Type: Endothelial Cell; Microvascular Cell

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Cat.No.
CSC-C8273W
Description
B129 Mouse Small Intestinal Microvascular Endothelial Cells from Creative Bioarray are isolated from small intestine of B129S2/SvPasCrl pathogen-free laboratory mice and grown in gelatin pre-coated cell culture flask with Creative Bioarray’ Complete Growth Medium. Cells at passage 3 are harvested and cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages under the cell culture conditions specified by Creative Bioarray.
Species
Mouse
Source
Small Intestine; Intestine
Cell Type
Endothelial Cell; Microvascular Cell
Disease
Normal
Quality Control
B129 Mouse Spleen Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R&D System) or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. B129 Mouse Spleen Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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