Human Stomach Smooth Muscle Cells

Description: Endothelial cells lining the microvasculature are known to play a critical "gatekeeper" role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that intestinal microvascular endothelial cells (IMEC) exhibit a strong immune response to LPS challenge and play a critical regulatory role in gut inflammation. Pharmacological inhibition of NOS in activated HIMEC resulted in a significant increase in leukocytes binding. Gene expression profile study reveals that intestinal endothelial cells express biotinidase, which is involved in biotin recycling. The in vitro culture of HIMEC enabled scientists to perform systematic analyses of the cytokine profiles with regard to mRNA expression and protein secretion, and to compare such data with cytokine profiles concomitantly displayed by other endothelial cells.HIMEC from Bioarray Research Laboratories are isolated from human intestinal tissue. HIMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HIMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HIMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIMEC are guaranteed to further culture in the conditions provided by Bioarray Research Laboratories.

Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn's disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.
HIF are isolated from human intestinal tissue. HIF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HIF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HIF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

Description: Smooth muscle is responsible for the contractility of hollow organs, such as blood vessels, the gastrointestinal tract, the bladder, and the uterus. Its structure differs greatly from that of skeletal muscle. The human stomach contains three layers of muscle in its walls, the outer longitudinal, the middle circular and the inner oblique and visceral smooth muscle cells makes up all three layers along the entire organ. Smooth muscle contraction is critical to peristalsis in the human stomach and the contraction may be mediated by activation of phospholipase through two distinct mechanisms (increased intracellular Ca2+ and G protein activation) and activating PKCepsilon-dependent mechanisms. In vitro study also shows that gastric smooth muscle cells express ET and eNOS and both calcium and sodium may be involved as current carriers in the generation of the plateau potential.HGSMC from Bioarray Research Laboratories are isolated from the human stomach. HGSMC are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HGSMC are characterized by immunofluorescent method with antibodies to Α-smooth muscle actin and desmin. HGSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGSMC are guaranteed to further expand for 15 population doublings at the condition provided by Bioarray Research Laboratories.

Description: Primary Human Intestinal Microvascular Endothelial Cells were initiated by elutriation from dissociated normal human small intestine tissue.
These cells were originated using Complete Serum-Free Medium Kit With SuperFuel™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5 X 10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.