Porcine Kidney Glomerular Endothelial Cells

Cat.No.: CSC-C8639W

Species: Porcine

Source: Kidney

Cell Type: Endothelial

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Cat.No.
CSC-C8639W
Description
Porcine Kidney Glomerular Endothelial Cells from Creative Bioarray are isolated from kidney glomerular tissue of porcine. Porcine Kidney Glomerular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Species
Porcine
Source
Kidney
Cell Type
Endothelial
Disease
Normal
Quality Control
Porcine Kidney Glomerular Endothelial Cells are tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Porcine Kidney Glomerular Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
Should DMSO be removed immediately when the cell frozen tubes are thawed and cultured?

Except for a few cells that are sensitive to DMSO, most of the cell lines (including suspension cells) should be placed directly into a culture corner bottle containing 10-15 ml of fresh medium after thawing, and then replaced with fresh medium the next day to remove DMSO.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

It gives outstanding results

The cell gave excellent results when we studied glomerulus-related diseases.

10 May 2023


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