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Human Renal Proximal Tubule Epithelial Cells-Diabetic Type II
Cat.No.: CSC-C8087L
Species: Human
Source: Kidney
Cell Type: Epithelial
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Diabetic Renal Proximal Tubule Epithelial Cells are isolated from human donors that have been diagnosed with diabetes type II disease. Further donor information, including medications, BMI, and disease duration may be obtained by contacting Scientific Support. Diseased cells are grown in the same optimized media system as Normal Human Renal Cells.
Applications:
Renal physiology
Diabetes Type II
Cancer
Cytokine production
Glomerulonephritis
Prostaglandin activity
Toxicology
Phagocytosis of immune complexes
Cellular function and differentiation
All cell types are guaranteed through 15 population doublings. RPTEC are guaranteed to test positive for gamma-GTP. HRE are guaranteed to test positive for pan cytokeratin. HRCE are guaranteed to stain positive for cytokeratin. NHMC are guaranteed to test positive for fibronectin and negative for cytokeratin 19 and von Willebrand Factor VIII. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots.
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Description: Creative Bioarray offers a normal human Renal Proximal Convoluted Tubule Epithelial cell system. Renal proximal convoluted tubule epithelial cells are an essential research tool for understanding a variety of biological processes in the kidneys. They can also be utilized to determine renal toxicity of compounds as well as to study underlying causes and fundamental aspects of human kidney diseases.
These primary RPCTs are collected from unfractionated kidney cell preparations from normal, non-diseased human kidney tissues. The cells can be cultured and expanded in Bioarray's unique renal epithelial cell culture media formulations delivering a robust renal cell culture for use in experiments and screening assays.
Bioarray provides RPCTs cryopreserved at 500,000 cells/vial along with 50ml of RPCT plating media for each vial to establish initial culture. Purity of each lot is verified using morphology and CD13 flow cytometry – Bioarray RPCTs are qualified at >80% CD13+ upon QC release.
Each lot of primary renal cells are tested and verified negative for HIV-1, HIV-2, HTLV I & II, Hep B & C, Syphillis and CMV.
Custom donor specifications are available upon request and a number of different plate and flask formats can be customized to meet your particular research project needs.
Description: HRPTEpiC from Creative Bioarray Research Laboratories are isolated from human kidney. HRPTEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRPTEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HRPTEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRPTEpiC are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray.
Description: HRMC from Creative Bioarray Research Laboratories are isolated from human renal tissue. HRMC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRMC are characterized by immunofluorescent method with antibodies to fibronectin, Thy-1, and smooth muscle actin. HRMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRMC are guaranteed to further expand for 15 population doublings at the conditions specified by Creative Bioarray.
Description: Human Renal Cortical Epithelial Cells (HRCECs) provided by Creative Bioarray are isolated from the normal human kidney tissue. The cells are cryopreserved at passage 2 and delivered frozen. Each vial contains more than 0.5*10^6 viable cells after thawing. The cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRCECs are guaranteed for at least 12 population doublings under the conditions provided by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Description: Creative Bioarray's normal Human Renal Epithelial Cells, when grown in Creative Bioarray's LIRen Medium, provide an ideal low-serum culture model for the study of renal function, metabolism, nephrotoxicity or cancer research. Creative Bioarray's Renal Proximal Tubule Epithelial Cells are cryopreserved as secondary cells to ensure the highest viability and plating efficiency. Our Renal Epithelial Cells are quality tested in LIRen Medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium.
Cell Features:
Mixed Renal Epithelial, Renal Cortical Epithelial, and Renal Medullary Epithelial are cryopreserved as primary cells isolated from human kidney tissue and expanded in culture vessels once before cryopreservation.
Renal Proximal Tubule Epithelial are cryopreserved as secondary cells isolated from human kidney tissue and expanded in culture vessels twice before cryopreservation.
All Renal Epithelial Cell types can be grown in a 0.5% serum medium without phenol red or antimicrobials when cultured in LIRen Medium.
All Renal Epithelial Cell types are extensively tested for quality and optimal performance.
Creative Bioarray guarantees performance and quality.
