Human Aortic Fibroblasts

Cat.No.: CSC-8238W

Species: Human

Source: Aorta

Morphology: Fibroblasts

Cell Type: Fibroblast

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Cat.No.

CSC-8238W

Description

Human Primary Aortic Fibroblasts are isolated from normal human aorta. Human Primary Aortic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml.

Species

Human

Source

Aorta

Morphology

Fibroblasts

Applications

Human Primary Aortic Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Cell Type

Fibroblast

Disease

Normal

Quality Control

Human Primary Aortic Fibroblast are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Human Primary Aortic Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

We ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.