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Human Alveolar Epithelial Cells

Cat.No.: CSC-C9223J

Species: Human

Source: Lung

Cell Type: Epithelial Cell

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Cat.No.
CSC-C9223J
Description
Human Alveolar Epithelial Cells from Creative Bioarray are isolated from normal human lung tissue. Human Alveolar Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Species
Human
Source
Lung
Recommended Medium
Human Complete Epithelial Cell Medium
Cell Type
Epithelial Cell
Disease
Normal
Storage and Shipping
We ship frozen cells on dry ice. Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

The mature alveolar epithelium consists of type I and type II alveolar epithelial cells (AECI and AECII), also known as type I and type II pneumocytes, which account for about 96% and 4% respectively of the surface, although their numbers are roughly similar. AECIs, which are membranous flat cells usually found overlying the capillaries, provide the thin surface of the alveolus. The AECIIs are large cuboidal cells located in the corners of the alveoli, with a characteristic morphology of lamellar bodies and apical microvilli.

Functions of Alveolar Epithelial Cells

Gas exchange - The most notable feature of the alveolar region is its considerable surface of contact between air and blood, which is required for the huge gas exchange function adapted to the human body's oxygen demand.

Barrier function - One of the major roles of the lung epithelium is to serve as a physical barrier between the lumen and the underlying submucosa, protecting the tissue from bacteria, virus, allergens, and other noxious substances.

Immunomodulatory function - In addition to their function of mechanical barrier against pathogens and various inhaled environmental materials, AECs participate actively in the immune response of the lung. From the numerous studies focused on the inflammatory response of the alveolar epithelium and the identification of the myriad of molecules that may be generated under various insult conditions, distinct roles of AECIs and AECIIs begin to emerge.

Alterations in the function of AECs are a hallmark of a large variety of acute and chronic lung diseases, including lung cancer. The function of these cells can be studied in human lung tissue, in animal models and in cell culture. Cell culture has the advantage that isolated cells can be exposed to disease-relevant stimuli under controlled conditions, and can be manipulated by a variety of techniques, including genetic editing.

DPP4 Inhibition Expands Human and Mouse AEC2s

To identify mechanisms for expanding AEC2s, we envisioned performing a high-content imaging screen, evaluating markers of proliferation in AEC2s in response to compound treatment. To overcome the technical difficulty of obtaining enough primary AEC2s to screen a large chemical library, we established a miniaturized screening assay using commercially available primary human small airway epithelial cells (SAECs). SAECs from preselected batches with high AEC2 signatures were used at early passage to best preserve high AEC2 character.

Consistent with the literature, the growth factors EGF, IGF-1, and FGF-10 promoted proliferation of this cell type. In addition, four structurally distinct FDA-approved gliptins- sitagliptin, saxagliptin, alogliptin, and linagliptin, as well as several clinical-stage DPP4 inhibitors induced a robust proliferative response in SAECs. Of these, the dipeptidyl peptidase 4 (DPP4) inhibitor, NVP-728, was the most active and displayed minimal cytotoxicity (Fig. 1).

Fig. 1: DPP4 inhibitor, NVP-728, promoted proliferation of AEC2s and displayed minimal cytotoxicity. Fig. 1. DPP4 inhibitors selectively expand AEC2s (Shao, Sida, et al. 2024).

Customized Culture Medium for In Vitro Growth of Human Alveolar Epithelial Cells (AECs)

The researchers defined a small-molecule cocktail (SMC) medium, which was supplemented with Jagged-1 peptide (JAG-1), recombinant human Noggin protein (hNoggin), SB431542, recombinant human fibroblast growth factor (hFGF)-10 protein, hFGF-7, CHIR99021, and Y-27632, as an initial step in designing a standard method for long-term 2D culture of AECs. They confirmed the morphological changes to identify the expression of the epithelial cell adhesion molecule (EpCAM) and Vimentin-specific markers of epithelial and mesenchymal cells, respectively-to determine whether the SMC medium supports in vitro growth of AECs (Fig. 2a). They also evaluated the necessity of various supplements in the SMC medium by removing single additives, i.e., –JAG-1, –hNoggin, –SB431542, –hFGF-10, –hFGF-7, –CHIR99021, and –Y-27632, to evaluate the necessity of various supplements in the SMC medium. The results provide important insights into the support of SMC medium for the in vitro culture of the AECs, which emphasizes the realization of the stable culture of AECs (Fig. 2).

Fig. 2. Customized human alveolar epithelial cell culture medium development.Fig. 2. Development of customized human alveolar epithelial cell culture medium (Yang, Jia-Wei, et al. 2022).

How long do you recommend further culture for? How many further doublings can be expected once received?

Human Alveolar Epithelial Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Is there any history of cancer?

No, the cells are isolated from healthy donor.

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