IPC-298

Cat.No.: CSC-C0352

Species: Human

Source: melanoma

Morphology: adherent, spindle-like cells (a few epitheliod cells) growing as confluent monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0352

Description

Established from the primary tumor (right cervical) of a 64-year-old woman with cutaneous melanoma

Species

Human

Source

melanoma

Recommended Medium

90-95% RPMI-1640 + 5-10% h.i. FBS

Culture Properties

monolayer

Morphology

adherent, spindle-like cells (a few epitheliod cells) growing as confluent monolayer

Karyotype

Human flat-moded hypertriploid karyotype with 13% polyploidy - 74(74-88)<3n>XX, -X, +1, -2, +3, -4, +6, +7, +9, -11, -11, +12, -13, -14, -15, +16, +21, +21, +4mar, dmin in 34% cells, del(3)(p14)x2, del(3)(q26.3)x2, del(6)(p23), del(7)(p14), add(7)(p22;HSR

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 +, neurofilament +, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.