SK-MEL-30

Cat.No.: CSC-C0302

Species: Human

Source: melanoma

Morphology: adherent, epithelial-like cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0302

Description

Derived from tumor tissue (subcutis metastasis) of a 67-year-old Caucasian man with malignant melanoma in 1975

Species

Human

Source

melanoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

adherent, epithelial-like cells growing as monolayers

STR DNA Profile

Amelogenin: X, Y
D5S818: 9, 12
D13S317: 8, 11
D7S820: 8, 9
D16S539: 8, 11
vWA: 15, 17
TH01: 6, 6
TPOX: 8, 11
CSF1PO: 10, 10
D19S433: 14, 15
D21S11: 29, 31.2
D18S51: 13, 15
D6S1043: 12
D3S1358: 15, 16
Penta D: 12
D2S441: 10
D8S1179 8, 14
Penta E: 5, 14
D12S391: 18, 21
D2S1338: 19, 21
FGA: 20, 21

Karyotype

Human hypertriploid karyotype with 3.5% polyploidy - 80(69-83)<3n>XXY-Y/XXYY, +1, +1, +2, +2, +3, +7, +10, -11, +12, +17, +18, +20, -21, +2mar, inv(6)(p11;q25), der(7)t(7;14)(p22;q22), der(9)t(9;?21)(p11;q11)x2, 14p+, der(15)t(15;?)(p12;?)x2, der(16)t(16;

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 +, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.