COLO-818

Cat.No.: CSC-C0237

Species: Human

Source: melanoma

Morphology: adherent growth in monolayers, mostly spindle-like, some cells with cellular processes, 1-5% very big cells, no strict contact inhibition

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Cat.No.

CSC-C0237

Description

Established from malignant melanoma

Species

Human

Source

melanoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Morphology

adherent growth in monolayers, mostly spindle-like, some cells with cellular processes, 1-5% very big cells, no strict contact inhibition

Karyotype

Human hypotriploid karyotype with 30% polyploidy - 62(57-63)<3n>X, -X, -X, -4, -5, +7, +8, -9, -10, -11, -13, +15, -16, -17, -19, -21, -21, +3mar, 1 dm present in 15% cells - add(3)(p11), der(13;21)(p10;10) - high level of subclonal rearrangement present

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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