RVH-421

Cat.No.: CSC-C0287

Species: Human

Source: melanoma

Morphology: adherent, epithelial-like cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0287

Description

Established from the tumor biopsy of an intracranial secondary melanoma in a 28-year-old European man in 1979; it was described that increasing the tyrosine concentration enhances synthesis of melanin

Species

Human

Source

melanoma

Recommended Medium

RPMI-1640 + 10% h.i. FBS.

Culture Properties

monolayer

Morphology

adherent, epithelial-like cells growing in monolayers

Karyotype

Human near-triploid karyotype with 30% polyploidy - 69(60-70)<3n>XXY, -10, +13, -15, +16, -20, +mar, del(6)(q15), add(8)(q24), del(9)(p22), der(9)t(7;9)(q12;q34)x2, add(11)(q24-25), ins(12;abr)(?q13), der(13)(t(6;13)(p13;q33)x2-3, add(19)(p13)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 +, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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