COLO-800

Cat.No.: CSC-C0319

Species: Human

Source: melanoma

Morphology: epithelial-like cells growing as monolayer; some cells look like fibroblasts or neurons

Culture Properties: monolayer

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Cat.No.

CSC-C0319

Description

Established from the subcutaneous nodule of a 14-year-old boy with melanoma in 1990

Species

Human

Source

melanoma

Recommended Medium

90-95% RPMI-1640 + 5-10% h.i. FBS

Culture Properties

monolayer

Morphology

epithelial-like cells growing as monolayer; some cells look like fibroblasts or neurons

Karyotype

Human hypotriploid karyotype with minor hypertriploid clone and 8% polyploidy - 64(50-69)<3n>X, -Y, -X/Y, -4, -5, +7, -8, -9, -10, -11, -12, +13, +13, +14, +15, -16, -19, +20, -21, -21, der(3)t(3;?)(p11;?), der(13q21q)x2, del(14)(?q11-12q24.2)x2, der(15)t

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 +, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.