SK-MEL-3

Cat.No.: CSC-C0099

Species: Homo sapiens (human)

Morphology: adherent epithelial cells growing as monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0099

Description

Established from the lymph node metastasis of a malignant melanoma from a 42-year-old Caucasian woman

Species

Homo sapiens (human)

Tissue of Origin

Skin

Recommended Medium

SuperCult® McCoys 5a Medium + 10% h.i. FBS

Culture Properties

monolayer

Morphology

adherent epithelial cells growing as monolayer

Karyotype

Human near-tetraploid karyotype with 3% polyploidy - 92(80-93)<4n>XX, -X, -X, -6, +7, +7, +7, -10, -10, -16, -16, +20, +20, +20, +20, +22, dup(7)(p15p22)x2, der(9)t(7;9)(p11;p21)dup(7)(p15p22)x2 - sideline with add(2)(p23) marker - resembles published kar

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, EpCAM -, GFAP -, HMB-45 +, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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