VM-CUB1

Cat.No.: CSC-C0446

Species: Human

Source: urinary bladder transitional cell carcinoma

Morphology: adherent epithelial-like cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0446

Description

Established from the primary transitional cell carcinoma of the urinary bladder of a male patient; described to carry a p53 point mutation

Species

Human

Source

urinary bladder transitional cell carcinoma

Recommended Medium

90% DMEM + 10% h.i.FBS

Culture Properties

monolayer

Morphology

adherent epithelial-like cells growing as monolayers

Karyotype

Human hypertriploid karyotype with 4% polyploidy - 80<3n>XX/XX?Y, +1, +1, +5, +6, +7, +9, -11, +15, -18, +19, +20, +22, +22, +7mar, del(1)(q21), del(1)(q11), der(1;6)(p10;q10)x2, add(3)(p21.3)x2, i(5p), der(6)t(1;6)(q11;q22), ?inv(7)(p15q35), i(9q), del(1

Quality Control

Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokera

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the grade of DMSO?

The grade of DMSO used for cryopreservation must be Tissueculturegrade DMSO, which is inherently sterile.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Technical helps

Gave me good technical help when my cells were suspected of being contaminated.

28 Aug 2022

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For research use only. Not for any other purpose.