BT-B

Cat.No.: CSC-C0334

Species: Human

Source: cervix carcinoma (derivative of HELA)

Morphology: epitheloid cells growing adherently in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0334

Description

These cells were thought to be "stablished from the malignant urinary bladder carcinoma of a 66-year-old Caucasian man (stage pTa G3) in 1989"; same donor as for cell line BT-A; however, DNA fingerprinting and cytogenetic analysis at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of HELA

Species

Human

Source

cervix carcinoma (derivative of HELA)

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

epitheloid cells growing adherently in monolayers

Karyotype

Human hypertriploid karyotype with 13% polyploidy - 72(68-74)<3n>XX, -Y, +1, -4, +5, +5, +5, -11, +12, +12, -19, -21, -22, +3mar, der(1)t(1;3)(p11;q11), der(1)t(1;5) (p21;q21), der(2)t(2;5)(p11;q11), del(3)(q13), add(4)(q35), del(5)(p12), i(5p)x3, add(6)(

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, endot

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the approximate cell viability?

Approximately 95% by trypan blue exclusion.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

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27 Apr 2021

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For research use only. Not for any other purpose.