TCC-SUP

Cat.No.: CSC-C0425

Species: Human

Source: urinary bladder transitional cell carcinoma

Morphology: epithelial-like adherent cells with smooth borders growing as monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0425

Description

Established from the tumor specimen resected from the urinary bladder transitional cell carcinoma (undifferentiated, grade IV) of a 67-year-old woman in 1974 (without therapy)

Species

Human

Source

urinary bladder transitional cell carcinoma

Recommended Medium

80-85%DMEM + 15-20% h.i.FBS

Culture Properties

monolayer

Morphology

epithelial-like adherent cells with smooth borders growing as monolayer

Karyotype

Human hypotriploid karyotype with 8% polyploidy - 67(58-67)<3n>XX, -X, +1, -2, -4, +5, -9, -12, -18, -21, +1-2mar, der(1;21)(q10;q10), del(1)(q13), i(5p)x3, del(6)(q14), add(6)(p23)/der(6)t(3;6)(p22;p22), add(6)(p22/23), del(10)(p11), add(20)(q12), add(21

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What cell concentration should be present in a cell freezing tube when cells are to be cryopreserved?

The number of cells in a cryotube is generally 1x10⁶cells / mlvial, while 5x10⁶cells / mlvial is appropriate for fusion tumour cells.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Successful subculture

The cell product I received was successfully subcultured.

04 Dec 2022

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For research use only. Not for any other purpose.