SBC-7

Cat.No.: CSC-C0336

Species: Human

Source: cervix carcinoma

Morphology: adherent epitheloid cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0336

Description

These cells were thought to be "established from the malignant urinary bladder carcinoma of a 82-year-old Caucasian woman (stage pTa G1-G2) in 1992"; same donor as for cell line SBC-2; however, DNA fingerprinting and cytogenetic analysis at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of HELA

Species

Human

Source

cervix carcinoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

adherent epitheloid cells growing in monolayers

Karyotype

Human hypertriploid karyotype with 15% polyploidy - 73/74(70-77)<3n>XX/XXX, +1, -4, +5, +6, -9, -9, +11, -13, -15, +16, +16, +17, +17, -22, -22, +4mar, der(1)t(1;14)(p11;q11), i(3q), del(3)(q11), add(5)(p11), i(7q), der(8)t(8;?11)(q24;q11), i(11p)x2, add(

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 (+), cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, end

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is included in the quality control report of the product?

Our QC report includes cell stock activity and value-added assays, microscopic examination of bacterial/fungal/mold contaminants, PCR assays for mycoplasma/chlamydia and others.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Thanks to the technician

Many thanks to the technician who gave me a lot of help with my experiments.

14 July 2021

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For research use only. Not for any other purpose.