BFTC-905

Cat.No.: CSC-C0413

Species: Human

Source: urinary bladder transitional cell carcinoma

Morphology: epitheloid cells growing adherently in monolayers, forming large islets

Culture Properties: monolayer

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Cat.No.

CSC-C0413

Description

Established from a 51-year-old Chinese woman with a grade III papillary transitional cell carinoma of the urinary bladder in 1990; patient lived in a blackfoot disease endemic area in Taiwan

Species

Human

Source

urinary bladder transitional cell carcinoma

Recommended Medium

80-90% DMEM+ 10-20% h.i.FBS

Culture Properties

monolayer

Morphology

epitheloid cells growing adherently in monolayers, forming large islets

Karyotype

Human flat-moded hyperdiploid/hypotriploid karyotype - 58(52-61)<3n>X/XX-X, -4, +5, -9, -13, -15, -16, -18, -21, -22, -22, +mar, add(1)(p36), i(5p), add(5)(q35), del(9)(p22), del(11)(p11), add(11)(q13), add(13)(p12), add(13)(p12), add(15)(p12), add(19)(p1

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays; bacterial contamination was eliminated with Baytril (enrofloxacin) and BM-Cyclin (tiamulin & minocycline)
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cy

Storage and Shipping

Frozen with 70% medium, 20% FBS and 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How do I keep the water tray of my CO₂ incubator clean?

Change it regularly (at least every fortnight) with sterile distilled water or sterile deionised water.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent reproducibility

The cell product has excellent reproducibility.

15 Nov 2021

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For research use only. Not for any other purpose.