SBC-2

Cat.No.: CSC-C0335

Species: Human

Source: cervix carcinoma

Morphology: epitheloid cells growing adherently in monolayers, often triangle-formed cells

Culture Properties: monolayer

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Cat.No.

CSC-C0335

Description

These cells were thought to be "established from the malignant urinary bladder carcinoma of a 82-year-old Caucasian woman (stage pTa G1-G2) in 1992"; same donor as for cell line SBC-7; however, DNA fingerprinting and cytogenetic analysis at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of HELA

Species

Human

Source

cervix carcinoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

epitheloid cells growing adherently in monolayers, often triangle-formed cells

Karyotype

Human hypertriploid karyotype with 2% polyploidy - 75(66-80)<3n>XX, -X, +1, +3, -4, +5, +6, +8, -9, +10, +11, +11, +12, -13, -14, -15, +16, +17, -18, -22, +3-5mar, del(3)(q11), der(3)t(1;3)(q11;q13), der(3)t(3;5)(q13;q11), i(5p), i(7p)x1-2, der(8)t(8;11)(

Quality Control

Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, end

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the growth condition of the cells?

The cells are grown in suspension as aggregates and only the cells in the aggregates are viable, but cell viability cannot be estimated and the general medium contains a large amount of cellular debris.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

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14 Feb 2022

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