3T3-L1 Preadipocytes

Cat.No.: CSC-7692W

Species: Mouse

Source: Adipose

Cell Type: Preadipocyte

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Cat.No.
CSC-7692W
Description
3T3-L1 murine adipocytes have been fundamental in metabolic disease research for over 30 years. Originally derived from Swiss mouse embryo tissue by Dr. Howard Green of Harvard Medical School, the 3T3-L1 system has been pivotal in advancing the understanding of basic cellular mechanisms associated with diabetes, obesity and related disorders.
We now offer both Cryopreserved and Plated sub-confluent 3T3-L1 preadipocytes in 96 well format and media for proliferation, differentiation, maintenance of 3T3-L1 preadipocytes to adipocytes. We also offer media and reagent kits validated using 3T3-L1 and/or primary rodent preadipocytes and adipocytes. High quality control tested 3T3-L1 preadipocytes designed to work consistently using Bioarray's line of 3T3-L1 media.
Species
Mouse
Source
Adipose
Cell Type
Preadipocyte
Disease
Normal
Storage and Shipping
Store in liquid nitrogen and ship in dry ice.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What media do I need to differentiate the cells?

In order to complete the differentiation process, you will need SuperCult® Preadipocyte Differentiation Medium Kit (cat# CM-1145X) and SuperCult® Adipocyte Medium (cat# CM-1230W), please order media according to your needs.

When do the cells differentiate?

Oil droplets should appear within 4-7 days after differentiation is induced. They look extremely small initially, continue to accumulate and gradually fuse into several big locules.

Do you provide ready to use plated 3T3-L1 adipocytes?

No. At this time they are too sensitive to the stresses of shipping during differentiation. Only cryopreserved and sub-confluent preadipocytes are provided as live plated cells.

What plated formats do you provide for 3T3-L1 preadipocytes?

We provide 3T3-L1 preadipocytes in the following formats: 96-well, 48-well, 24-well, 8-chamber slides.

Why don't my 3T3-L1 Preadipocytes differentiate well?

Use cells of a lower passage number. The 3T3-L1 cell line is NOT immortalized and is suitable until passage 12-13. Ensure cells are 100% confluent for 48 hours prior to initiating differentiation. Do not use fetal bovine serum during the proliferation process. It will affect later differentiation potential. We recommend using Creative Bioarray’s SuperCult® Preadipocyte Differentiation Medium Kit (cat# CM-1145X).

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Successful subculture

The cell product I received was successfully subcultured.

13 May 2022


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