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3T3-L1 Preadipocytes
Cat.No.: CSC-7692W
Species: Mouse
Source: Adipose
Cell Type: Preadipocyte
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We now offer both Cryopreserved and Plated sub-confluent 3T3-L1 preadipocytes in 96 well format and media for proliferation, differentiation, maintenance of 3T3-L1 preadipocytes to adipocytes. We also offer media and reagent kits validated using 3T3-L1 and/or primary rodent preadipocytes and adipocytes. High quality control tested 3T3-L1 preadipocytes designed to work consistently using Bioarray's line of 3T3-L1 media.
In order to complete the differentiation process, you will need SuperCult® Preadipocyte Differentiation Medium Kit (cat# CM-1145X) and SuperCult® Adipocyte Medium (cat# CM-1230W), please order media according to your needs.
Oil droplets should appear within 4-7 days after differentiation is induced. They look extremely small initially, continue to accumulate and gradually fuse into several big locules.
No. At this time they are too sensitive to the stresses of shipping during differentiation. Only cryopreserved and sub-confluent preadipocytes are provided as live plated cells.
We provide 3T3-L1 preadipocytes in the following formats: 96-well, 48-well, 24-well, 8-chamber slides.
Use cells of a lower passage number. The 3T3-L1 cell line is NOT immortalized and is suitable until passage 12-13. Ensure cells are 100% confluent for 48 hours prior to initiating differentiation. Do not use fetal bovine serum during the proliferation process. It will affect later differentiation potential. We recommend using Creative Bioarray’s SuperCult® Preadipocyte Differentiation Medium Kit (cat# CM-1145X).
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Average Rating: 5.0 | 1 Scientist has reviewed this product
Successful subculture
The cell product I received was successfully subcultured.
13 May 2022
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