CAL-51

Cat.No.: CSC-C0382

Species: Human

Source: breast carcinoma

Morphology: epithelial-like adherent cells growing in monolayers; the culture contains usually a large amount of cell debris

Culture Properties: monolayer

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Cat.No.

CSC-C0382

Description

Established from the pleural effusion metastasis of a 45-year-old woman with progressive breast adenocarcinoma (after radio-, chemotherapy and surgery) in 1985; rare example of tumor cell line with normal karyotype

Species

Human

Source

breast carcinoma

Recommended Medium

80% DMEM + 20% h.i.FBS

Culture Properties

monolayer

Morphology

epithelial-like adherent cells growing in monolayers; the culture contains usually a large amount of cell debris

Karyotype

Human near-diploid karyotype with 28% tetraploidy - 46<2n>XX, no consistent abnormality detected - rare example of tumor cell line with normal karyotype - resembles published karyotype

Reference

Publication

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vime

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What causes viral contamination in cell culture?

The virus may come from the serum.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Competent platform

Creative Bioarray is a very competent and professional platform.

16 May 2021

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