HCEC-B4G12

Cat.No.: CSC-C3457

Species: Human

Source: corneal endothelium

Morphology: cuboid epitheloid cells growing strongly adherent in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C3457

Description

HCEC-B4G12 is a clonal subpopulation started in 2005 from the parental cell line HCEC-12 established from normal cells of the posterior epithelium of the cornea of a 91-year-old Caucasian woman transformed with the early region of the SV40 genome including genes encoding large T-antigen and small t-antigen; the subclone is described to represent differentiated cells of the so-called corneal endothelium

Species

Human

Source

corneal endothelium

Recommended Medium

Human-Endothelial-SFM + 10 ng/ml FGF-2 (observe the shelf-life of the medium; do not apply h.i. FBS in order to keep differentiation status)

Culture Properties

monolayer

Morphology

cuboid epitheloid cells growing strongly adherent in monolayers

Reference

Publication

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 (+), cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Viruses: P

Storage and Shipping

Frozen with 90% medium (including FGF-2), 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How are quality control metrics and benchmarks established and monitored in cell biology?

Metrics such as cell viability, confluency, passage number, and contamination rates are routinely monitored to establish benchmarks for quality control in cell biology experiments.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Promising results

We were able to use this tumor cell product to test the efficacy of various cancer treatments, with promising results.

29 Mar 2023

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For research use only. Not for any other purpose.