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GCaMP3-Expressing Human Umbilical Vein Endothelial Cells (GCaMP3-HUVECs)
Cat.No.: CSC-C5423W
Species: Human
Source: Umbilical Cord; Vein
Cell Type: Endothelial Cell
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Cat.No.
CSC-C5423W
Description
GCaMP3 is a synthetic fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light-chain kinase. When bound to Ca2+, GCaMP3 fluoresces green with a peak excitation wavelength of 480 nm and a peak emission wavelength of 510 nm. It is used in biological research to measure intracellular Ca2+ levels both in vitro and in vivo using virally transfected or transgenic cell and animal lines.
GCaMP3 Expressing Human Umbilical Vein Endothelial Cells (GCaMP3-HUVECs) provided by Creative Bioarray are isolated from the umbilical vein tissue of human. These cells are puromycin selected from Human Umbilical Vein Endothelial Cells infected with lentivirus expressing GCaMP3. GCaMP3-HUVECs are cryopreserved in passage 3 and guaranteed for at least 18 population doublings under the cell culture conditions specified by Creative Bioarray. Each vial contains at leat 0.5 million cells. These cells are characterized by immunofluorescence staining with antibody of VWF/Factor VIII and PECAM1.These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C.
GCaMP3 Expressing Human Umbilical Vein Endothelial Cells (GCaMP3-HUVECs) provided by Creative Bioarray are isolated from the umbilical vein tissue of human. These cells are puromycin selected from Human Umbilical Vein Endothelial Cells infected with lentivirus expressing GCaMP3. GCaMP3-HUVECs are cryopreserved in passage 3 and guaranteed for at least 18 population doublings under the cell culture conditions specified by Creative Bioarray. Each vial contains at leat 0.5 million cells. These cells are characterized by immunofluorescence staining with antibody of VWF/Factor VIII and PECAM1.These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C.
Species
Human
Source
Umbilical Cord; Vein
Recommended Medium
Size
> 0.5 million cells/frozen vial
Cell Type
Endothelial Cell
Storage
Liquid Nitrogen
Shipping
Dry Ice
Quality Control
These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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