LCL-WEI

Cat.No.: CSC-C0332

Species: Human

Source: B lymphoblastoid cells

Morphology: lymphoblastoid cells growing singly and in large clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0332

Description

Epstein-Barr virus-positive cell line established from peripheral blood in 1977 from male patient with melanoma

Species

Human

Source

B lymphoblastoid cells

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblastoid cells growing singly and in large clumps in suspension

Karyotype

Human near-tetraploid karyotype with diploid (36%) sideline and 4% polyploidy - 90(88-94)<4n>XX/XXY/XXYY - no consistent structural aberrations

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD37 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -/+, sm

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.