LCL-HO

Cat.No.: CSC-C3441

Species: Human

Source: B lymphoblastoid cells

Morphology: lymphoblastoid-like cells growing in suspension singly or in clumps

Culture Properties: suspension

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Cat.No.

CSC-C3441

Description

This lymphoblastoid cell line was established by EBV transformation of peripheral blood lymphocytes; cell line was submitted as autologous to cell line MEL-HO that was derived from a woman with primary melanoma in 1976; however, cytogenetic and DNA fingerprinting analysis showed clearly that LCL-HO and MEL-HO are not related

Species

Human

Source

B lymphoblastoid cells

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblastoid-like cells growing in suspension singly or in clumps

Karyotype

Human diploid karyotype with 2% polyploidy and near triploid sideline (4%) - 46(45-47)<2n>XX, der(12)t(12;?6)(q24.32;p21.2) - additional pseudodiploid subclones are present with other structural and numerical rearrangements

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cy IgM -, cykappa -, cylambda +
Viruses: ELISA: reverse

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.