SIMA

Cat.No.: CSC-C3438

Species: Human

Source: neuroblastoma

Morphology: cell shape is bipolar, cells growing as monolayer or forming foci

Culture Properties: monolayer

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Cat.No.

CSC-C3438

Description

Established from the adrenal tumor tissue resected after treatment from a 20-month-old boy of European origin with neuroblastoma (stage III) in 1991

Species

Human

Source

neuroblastoma

Recommended Medium

90% RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

cell shape is bipolar, cells growing as monolayer or forming foci

Karyotype

Human hypotetraploid karyotype with 3.5% polypoidy - 88/89<4n>XXY/XXYY, -Y, -11, -17, -17, der(1qh++)t(1;17)(p34-35;q12)x2, t(6;9)(p21;q33)x2, ?inv(12)(q21q24)x2, der(22)t(17;22)(q21;p12)x2 - ca. 50-100 NMYC-containing dmin present in each cell - t(1;17)

Quality Control

Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +
Viruses: ELISA: reve

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why should the medium be washed with PBS in cell culture?

The pH value of PBS buffer is between 7.2 and 7.4, which is the most suitable pH range for cells. And the pH value will remain largely unchanged under stimulation by trace amounts of acid and base.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Good quality control

The quality control of the cell products is very good, as demonstrated by the good reproducibility and the ease of handling steps.

13 Feb 2021

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For research use only. Not for any other purpose.