GAMG

Cat.No.: CSC-C0345

Species: Human

Source: glioma

Morphology: adherent, large, spindle-like cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0345

Description

Established from the biopsy specimens from a 42-year-old woman with glioma (histologically defined as glioblastoma)

Species

Human

Source

glioma

Recommended Medium

90% DMEM + 10% h.i.FBS + 4x non-essential amino acids

Culture Properties

monolayer

Morphology

adherent, large, spindle-like cells growing in monolayers

Karyotype

Human hypotriploid karyotype with 17% polyploidy - 66(61-67)<3n>XXX, -4, +5, +7, -10, -13, -19, -22, +dm x1-2, der(1)t(1;4)(q11;p11), del(3)(p14), der(10)t(3;10)(p14;q13), del(17)(p11), del(18)(p11) - additional subclonal rearrangements, add(2)(q11), add(

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Do the cells need to be washed with PBS during changing the fluid?

In general, cells can be changed without PBS; however, if the cells are used for transfection, protein extraction and so on, the cells need to be washed with PBS.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Good performation

Our laboratory has purchased the GAMG for scientific research and the cells are performing well.

15 Oct 2021

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For research use only. Not for any other purpose.