MHH-NB-11

Cat.No.: CSC-C0304

Species: Human

Source: neuroblastoma

Morphology: adherent, fibroblast-like cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0304

Description

Established from a neuroblastoma metastasis at an adrenal site of a 4-year-old white boy in 1986; cells were described to express neuron-specific enolase and synaptophysin (but neither GFAP nor S-100 protein) and to carry a 20-fold amplification of MYCN (NMYC, N-myc)

Species

Human

Source

neuroblastoma

Recommended Medium

RPMI-1640 + 10% h.i. FBS + 1% non-essential amino acids

Culture Properties

monolayer

Morphology

adherent, fibroblast-like cells growing as monolayers

Karyotype

Human bimodal hyper-, diploid/tetraploid karyotype - 54(48-54)<2n>XY, +1, +1, +2, +6, +7, +8, +13, +20, der(1)t(1;?)(p32;?)x2, der(13)t(13;?2HSR)(q33;?2p23) - both the long HSR on ch 13 due to MYCN (NMYC, N-myc) amplification and the ch 1 short-arm rearra

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +
Viruses: ELISA: reve

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the purpose of using DPBS buffer for cell washing?

To avoid cell clumping, maintain cellular osmolarity balance, maintain pH stability and provide a relatively stable ionic environment for cells and tissues.

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