NBL-S

Cat.No.: CSC-C0638

Species: Human

Source: neuroblastoma

Morphology: polymorph cells growing adherently in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0638

Description

Established from the primary adrenal tumor resected prior to treatment from a 3-year-old boy with rapidly progressing Stage III neuroblastoma in 1989; described to lack MYCN (NMYC, N-myc) amplification and chromosome 1p deletions, but to show prolonged N-myc protein half life

Species

Human

Source

neuroblastoma

Recommended Medium

90% SuperCult® IMDM + 10% h.i. FBS

Culture Properties

monolayer

Morphology

polymorph cells growing adherently in monolayers

Quality Control

Mycoplasma: contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, EpCAM -, GFAP -, neurofila

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

When to stop trypsin digestion in cell culture?

Generally when you observe the adnexal cell layer with the naked eye, as soon as it can move, mostly in a sandy pattern, it is time to stop digestion.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Trustworthy

We have been using Creative Bioarray's cellular products and look forward to continuing to develop new ones.

22 Mar 2021

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For research use only. Not for any other purpose.