LAN-1

Cat.No.: CSC-C0637

Species: Human

Source: neuroblastoma

Morphology: cells are growing adherently and in clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0637

Description

Established in 1977 from a bone marrow metastasis of a 2-year-old boy with stage IV neuroblastoma

Species

Human

Source

neuroblastoma

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS or 80-90% EMEM + 10-20% h.i. FBS + non-essential amino acids + 2 mM L-glutamine

Culture Properties

suspension

Morphology

cells are growing adherently and in clumps in suspension

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, E

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the factors that affect the effectiveness of trypsin digestion?

The effectiveness of trypsin digestion is mainly related to pH, temperature, concentration of trypsin, size and hardness of the tissue mass.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Trustworthy

Many people around us are using Creative Bioarray products and we all agree that the quality of the products is good and trustworthy.

19 Apr 2022

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For research use only. Not for any other purpose.