LA1-5s

Cat.No.: CSC-C9416J

Species: Human

Specification
Q & A
Customer Review

Cat.No.

CSC-C9416J

Description

LA1-5s is a clonal subline of the cell line LA-N-1 which was established by Seeger et al., (1977) from the primary site of a noncatecholamine-producing neuroblastoma of a 3-year-old female with clinical Stage IV neuroblastoma. The LA1-5s culture consists of large, flat, and tightly adherent cells. They are precursors of Schwann cell/glia/melanocyte lineages arising from the neural crest. This cell line is not tumourigenic in nude mice at a 1,000,000 cell inoculum. The cells can transdifferentiate to a neuroblastic phenotype. The N-myc gene has been reported to be amplified but exhibits very low expression.LA-N-1, LA1-55n and LA1-5s have been shown to originate from the same patient by STR profiling.

Species

Human

Tissue of Origin

Neural

Recommended Medium

EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Storage

Liquid Nitrogen (-180 °C).

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What should I do if I find bacterial contamination during cell culture?

Generally, 5-10 times the usual amount of antibiotics is used as shock therapy and the culture is changed 24-48 hours later.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

Reliable products

We have always relied on Creative Bioarray's products because they have helped us to complete our experiments well.

17 July 2022

Ease of use
After sales services
Value for money

Write your own review

IMR-5

Description: Species: human, Caucasian;
Tumor: neuroblastoma

Cat#: CSC-6268W INQUIRY

View Product

SK-N-F1

Description: Species: human male;
Tumor: neuroblastoma;
Derived from: bone marrow metastasis

Cat#: CSC-6314W INQUIRY

View Product

U87/DK

Description: Species: human;
Tissue: brain;
Tumor: glioblastoma;
Transfected with binase (ATP binding site 721), mutated de 2-7 EGFR

Cat#: CSC-6323W INQUIRY

View Product

U87/WT

Description: Species: human;
Tissue: brain;
Tumor: glioblastoma;
Transfected with: Transfected with wt EGFR

Cat#: CSC-6324W INQUIRY

View Product

MHH-NB-11

Description: Established from a neuroblastoma metastasis at an adrenal site of a 4-year-old white boy in 1986; cells were described to express neuron-specific enolase and synaptophysin (but neither GFAP nor S-100 protein) and to carry a 20-fold amplification of MYCN (NMYC, N-myc)

Cat#: CSC-C0304 INQUIRY

View Product

GAMG

Description: Established from the biopsy specimens from a 42-year-old woman with glioma (histologically defined as glioblastoma)

Cat#: CSC-C0345 INQUIRY

View Product

For research use only. Not for any other purpose.