BE(2)-M17

Cat.No.: CSC-C9367J

Species: Human

Specification
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Cat.No.

CSC-C9367J

Description

The cell line BE(2)-M17 was isolated from SK-N-BE(2) by limiting dilution. The cells show a population doubling time of 20-24 hours, grow in multi-layers but start to aggregate and float as the culture ages. The neuroblastic cells express properties of neuroadrenergic neurones and multiple, moderately long cell processes. BE(2)-M17 showed tyrosine hydroxylase and dopamine-ß-hydroxylase activity and specific uptake of norepinephrine (noradrenalin). Expression of intermediate filaments was detected as well as neuron-specific enolase and moderate expression of vimentin. BE(2)-M17 are tumorigenic in nude mice.

Species

Human

Tissue of Origin

neural

Recommended Medium

EMEM (EBSS):Ham's F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS) (Heat Inactivated).

Storage

Liquid Nitrogen (-180 °C).

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What happens to the culture medium after bacterial contamination during cell culture?

After contamination, the medium changes from red to yellow for a short period of time due to the production of large amounts of acid. In addition, the medium becomes cloudy and floating material can be seen on the surface of the medium after a slight shaking.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Good growth

The cells are in good growth condition when observed through a microscope.

14 July 2022

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For research use only. Not for any other purpose.