ActoFactor™ Recombinant Rat Ciliary neurotrophic factor

Specification

Cat.No.

CSC-CTK0864

Description

Recombinant Rat Ciliary Neutrophic Factor (rRa CNTF) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. More recently, CNTF has been shown to promote survivability and differentiation of other neuronal cell types. rRa CNTF is a 22.7 kDa protein containing 200 amino acid residues.

Species

Rat

Product Overview

Rat Cntf expressed in E.coli

Molecular Mass

22.7 kDa

Size

CAT# CSC-CTK0864-25 (25 μg); CAT# CSC-CTK0864-125 (125 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE analysis.

Activity

The ED50 was determined by the dose-dependent proliferation of human TF-1 cells. The concentration range for this effect was 25.0 – 35.0 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from 5mM NaAcetate, pH 6.5.

Reconstitution

Please centrifuge product before opening vial. The lyophilized protein should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. Reconstituted rRa CNTF should be stored in working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

Q & A
Customer Review

Ask a Question

Write your own review

ONLINE INQUIRY