ActoFactor™ Recombinant Rat Chemokine (C-X-C motif) ligand 1

Specification

Cat.No.

CSC-CTK0856

Description

All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant rat GRO/KC is a 7.8 kDa protein consisting of 72 amino acids including the ELR motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

Species

Rat

Product Overview

Rat Cxcl1 expressed in E.coli

Molecular Mass

7.8 kDa

Size

CAT# CSC-CTK0856-25 (25 μg); CAT# CSC-CTK0856-125 (125 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

Determined by its ability to chemoattract rat neutrophils using a concentration range of 10-100 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

The protein was lyophilized with no additives.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa GRO should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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