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Cat.No.: CSC-C9423J

Species: Human

Specification
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Cat.No.

CSC-C9423J

Description

A human A human glioblastoma multiforme cell line derived from a 65-year-old male. The results of immunocytochemical analysis for GFAP, Vimentin, NestiN and CD33 were positive in early and late passage cells. MGMT methylation was negative. Karyotype: Near tetraploid; range 88 to 91 with two copies of marker: der(6;7;12;)del(6q ), t(6;11), t(6;17) del(17q). Six copies of chromosome 7 and other rearrangements with loss and gain of chromosome fragments involving chromosomes 1,2,3,5,10,9,19,20. The X and Y chromosomes are present.

Species

Human

Tissue of Origin

Brain

Recommended Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Storage

Liquid Nitrogen (-180 °C).

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Is it useful to use antibiotics after bacterial contamination has occurred during cell culture?

Antibiotics are mostly used in cell culture to prevent bacterial contamination, and half of them are often used in combination. It is often difficult to eradicate antibiotics once contamination has occurred, and some antibiotics have only an antibacterial but not a bactericidal effect.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Reduced contamination

I change the culture medium promptly during the cultivation of the cells, which greatly reduces the probability of contamination.

13 Apr 2022

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For research use only. Not for any other purpose.