647-V

Cat.No.: CSC-C0457

Species: Human

Source: urothelial bladder carcinoma

Morphology: adherent epithelial-like cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0457

Description

Established from a male patient with a primary urothelial bladder carcinoma (malignant, grade 2)

Species

Human

Source

urothelial bladder carcinoma

Recommended Medium

85% DMEM + 15% h.i.FBS

Culture Properties

monolayer

Morphology

adherent epithelial-like cells growing in monolayers

Karyotype

Human flat-moded hyperdiploid/hypotriploid karyotype with 12% polyploidy - 52-60<3n>XX, -X/Y, -2, -4, -6, -9, -10, -12, -12, -14, -15, -16, -17, -18, +5mar, del(1)(p32), del(2)(q23)/add(2)(q2?), add(3)(q27), add(3)(q1?), i(5p), i(6p), i(7p)x2, add(8)(q24)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What does the cell shape look like under the electron microscope?

Different numbers of rough endoplasmic reticulum and prominent microfilaments can be observed，which contains ras oncogenes.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Good growth

The cells grew in good condition and helped our experiments.

12 Dec 2021

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For research use only. Not for any other purpose.