42-MG-BA

Cat.No.: CSC-C0466

Species: Human

Source: glioma

Morphology: flat epithelial-like polygonal cells growing adherently as monolayer or, if confluent, as multilayers with processes

Culture Properties: monolayer

Inquire for Price

Specification
Q & A
Customer Review
Publications

Cat.No.

CSC-C0466

Description

Established from the temporal lobe brain biopsy from a 63-year-old man with glioblastoma multiforme in 1990 (prior to treatment)

Species

Human

Source

glioma

Recommended Medium

80% mixture of RPMI-1640 + EMEM (at 1:1) + 20% h.i. FBS

Culture Properties

monolayer

Morphology

flat epithelial-like polygonal cells growing adherently as monolayer or, if confluent, as multilayers with processes

Karyotype

Human flat-moded hypertetraploid karyotype with 8% polyploidy - 88-95<4n>XX, -Y, -Y, +4, -5, -5, -7, -8, -9, -9, +11, +11, -13, -13, +14, +14, +15, -16, -17, -17, -18, -18, -19, -21, -22, +14mar, add(1)(p1?), del(1)(q21.2), del(2)(q13)x2, dup(2)(p12p2?4)a

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Viruses: ELISA: revers

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the storage and transport environment for the cells?

Stored in liquid nitrogen and transported on dry ice.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

Helpful

I have learnt a lot from the Creative Bioarray website and it has been very helpful for my experiments.

29 Mar 2022

Ease of use
After sales services
Value for money

Write your own review

You May Also Need

CM-1041X

SuperCult® EMEM

INQUIRY

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

IMR-5

Description: Species: human, Caucasian;
Tumor: neuroblastoma

Cat#: CSC-6268W INQUIRY

View Product

SK-N-F1

Description: Species: human male;
Tumor: neuroblastoma;
Derived from: bone marrow metastasis

Cat#: CSC-6314W INQUIRY

View Product

U87/DK

Description: Species: human;
Tissue: brain;
Tumor: glioblastoma;
Transfected with binase (ATP binding site 721), mutated de 2-7 EGFR

Cat#: CSC-6323W INQUIRY

View Product

U87/WT

Description: Species: human;
Tissue: brain;
Tumor: glioblastoma;
Transfected with: Transfected with wt EGFR

Cat#: CSC-6324W INQUIRY

View Product

MHH-NB-11

Description: Established from a neuroblastoma metastasis at an adrenal site of a 4-year-old white boy in 1986; cells were described to express neuron-specific enolase and synaptophysin (but neither GFAP nor S-100 protein) and to carry a 20-fold amplification of MYCN (NMYC, N-myc)

Cat#: CSC-C0304 INQUIRY

View Product

GAMG

Description: Established from the biopsy specimens from a 42-year-old woman with glioma (histologically defined as glioblastoma)

Cat#: CSC-C0345 INQUIRY

View Product

For research use only. Not for any other purpose.