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Immortalized Rat Cardiac Microvascular Endothelial Cells-SV40 (Tet-on)
Cat.No.: CSC-I1914Z
Species: Rat
Morphology: Polygonal
Culture Properties: Adherent
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Cat.No.
CSC-I1914Z
Description
Rat Cardiac Microvascular Endothelial Cells were isolated and transfected with SV40LT expressing lentiviral particles under the control of a Tet-on system in the presence of DOX.
Species
Rat
Recommended Medium
SuperCult? Immortalized Rat Cardiac Microvascular Endothelial Cell Medium (Cat No.: CM-I1914Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
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Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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