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Immortalized Rat Cardiac Microvascular Endothelial Cells-SV40 (Tet-on)

Cat.No.: CSC-I1914Z

Species: Rat

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I1914Z
Description
Rat Cardiac Microvascular Endothelial Cells were isolated and transfected with SV40LT expressing lentiviral particles under the control of a Tet-on system in the presence of DOX.
Species
Rat
Recommended Medium
SuperCult? Immortalized Rat Cardiac Microvascular Endothelial Cell Medium (Cat No.: CM-I1914Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Recommended Products
CSC-C2124 Rat Cardiac Microvascular Endothelial Cells
CIK-HT003 HT? Lenti-SV40T Immortalization Kit
CIK-HT004 HT? Lenti-SV40 (tsA58 temperature sensitive mutant) Lentivirus Immortalization Kit
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.