Description
Immortalized Human Umbilical Vein Endothelial Cells-hTERT were developed from human tissues transduced with a lentiviral expression vector containing the hTERT gene. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence. The Immortalized Human Umbilical Vein Endothelial Cells are useful for setting up in vitro bioassays for studying angiogenesis in e.g. wound healing or metastasis as well as bioassays for testing the angiogenic properties of novel drugs. Additionally, the cell line is the perfect starting material for genetic engineering to create important disease models.
Recommended Medium
SuperCult® Immortalized Human Umbilical Vein Endothelial Cell Medium (Cat No.: CM-I9007L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
Human telomerase reverse transcriptase (hTERT)
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Recommended Products
CSC-C4076X HUVEC;Human Umbilical Vein Endothelial Cells
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Citation Guidance
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What is the principle of the telomerase transfection method?
Telomerase activation is a common step in various cell immortalization methods. By expressing human telomerase re-verse transcriptase (hTERT), telomerase activity can be activated, telomere length can be maintained, chromosome telomere degradation can be prevented, and epithelial cell immortalization can be induced. The immortalized cells established by telomerase transfection can maintain the physiological characteristics of normal cells.