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Immortalized Human Microglia-SV40
Cat.No.: CSC-I9004L
Species: homo sapiens
Source: Brain
Morphology: Polygonal
Culture Properties: Adherent
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Note: Never can cells be kept at -20 °C.
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
If most of the cells are attached back to the bottom of the bottle, it shows that the cell viability is normal, the remaining floating cells can be removed, leaving 8-10 mL culture medium culture observation, cell growth to confluence 85-95%, digestion and passaging, such as the cells are still not attached to the wall, the cells will be centrifuged and collected to a new culture bottle, the original bottle plus part of the medium to continue to cultivate, in the middle of the attention to observation, our technicians will be followed up and guidance until the problem is solved, and then the cells can be transferred back to the bottom of the bottle. Our technicians will follow up and guide until the problem is solved.
The Immortalized Human Microglia-SV40 cell line (CSC-I9004L) is derived from Primary Human Microglial Cells (>99% purity) and have maintained microglial specific markers such as Iba1, CD68, TREM2 and CD11b. These cells are suitable for studies of human
microglia in health and disease.
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Average Rating: 5.0 | 1 Scientist has reviewed this product
Reduced contamination
I change the culture medium promptly during the cultivation of the cells, which greatly reduces the probability of contamination.
13 Apr 2022
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Description: Immortalized Human Ovarian Epithelial Cells-SV40 have been obtained immortalizing Human Primary Ovarian Epithelial Cells with LentiSV40 Lentivirus. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40tranduced cells go beyond 30 passages.
