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Immortalized Human Umbilical Vein Endothelial Cells (SV40)-RFP

Cat.No.: CSC-I2046Z

Species: homo sapiens

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I2046Z
Description
Primary human umbilical vein endothelial cells were isolated and transfected with SV40LT and RFP expressing lentiviral particles. Immortalized Human Umbilical Vein Endothelial Cells (SV40)-RFP from Creative Bioarray go beyond 30 passages.
Species
homo sapiens
Recommended Medium
SuperCult® Immortalized Human Umbilical Vein Endothelial Cell Medium (Cat No.: CM-I2045Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.