Description
Immortalized Human Synovial Membrane Fibroblasts-SV40 were transfected with SV40LT expressing lentiviral particles. These primary cells go into senescence after the 2nd passage while the Immortalized Human Synovial Membrane Fibroblasts-SV40 go beyond 30 passages.
Recommended Medium
SuperCult® Immortalized Human Synovial Membrane Fibroblast Medium (Cat No.: CM-I9106L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Recommended Products
CSC-C9339W Human Synovial Membrane Fibroblasts
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What are Immortalized Human Synovial Membrane Fibroblasts-SV40?
Immortalized Human Synovial Membrane Fibroblasts-SV40 are cultured human fibroblasts derived from the synovial membrane, which lines joints. These cells have been genetically modified to express the SV40 (Simian Virus 40) large T antigen, allowing them to proliferate in vitro. They serve as an important model for studying joint diseases and inflammatory conditions.