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Immortalized Human Skin Fibroblast-SV40

Cat.No.: CSC-I9011L

Species: homo sapiens

Source: Skin

Morphology: Bipolar

Culture Properties: Adherent

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Cat.No.
CSC-I9011L
Description
Immortalized Human Skin Fibroblasts-SV40 have been obtained immortalizing Human Skin Fibroblasts with SV40 gene. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 4th passage while the SV40 gene‐tranduced cells go beyond 20 passages. This cell line is a great in vitro model system.
Species
homo sapiens
Source
Skin
Recommended Medium
SuperCult® Immortalized Human Skin Fibroblast Medium (Cat No.: CM-I9011L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Bipolar
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-7856W Human Skin Cells
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
How to resuscitate cells?

Thaw frozen tubes containing 1 mL of cell suspension by rapid shaking in a 37℃ water bath, add 5 mL of culture medium and mix well. Centrifuge at 1000 rpm for 5 minutes, discard the supernatant, replenish 4-6 mL of complete medium and blow well. All cell suspensions are then added to culture flasks and incubated overnight (or cell suspensions are added to 6 cm dishes) and incubated overnight. The next day, the solution is changed and the cell density is checked.

What research applications are Immortalized Human Skin Fibroblasts-SV40 suitable for?

Immortalized Human Skin Fibroblasts-SV40 are ideal for studying skin biology, wound healing, fibrosis, drug screening, and cosmetic testing. They provide a reliable and scalable model for testing and research in dermatological sciences.

Do you offer custom cell line development or services?

Yes, we offer custom cell line development tailored to meet unique research requirements. Please contact us to discuss your specific needs and learn more about our custom solutions.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Professional

Creative Bioarray is a professional in the field of cells and has helped me a lot.

12 Feb 2021


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For research use only. Not for any other purpose.